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NETQUANTを用いた好中球細胞トラップの自動画像ベース定量
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
DOI:

07:33 min

November 27, 2019

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Chapters

  • 00:04Title
  • 00:34Analysis and Quantification of Neutrophil Extracellular Traps (NETs)
  • 02:19Segmentation of Cells in the DNA Channel and the NET Channel
  • 03:41Single-cell Analysis of Identifiable Properties
  • 04:19Comparison of Cell Properties to Identify NET-forming Cells
  • 05:23Result Outputs Demeanor
  • 06:16Results: Analysis of NET Formation
  • 07:07Conclusion

Summary

Automatic Translation

ここでは、好中球細胞外トラップ(NET)を生成し、NETQUANTを動作させるプロトコルを提示し、免疫蛍光画像におけるNETの定量のための全自動ソフトウェアオプションを提示する。

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