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填充海马 ca2 间神经元的生物环素恢复与三维重建

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons

Please note that all translations are automatically generated. Click here for the English version.

填充海马 ca2 间神经元的生物环素恢复与三维重建

DOI:

10.3791/58592-v

•

11:21 min

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November 20, 2018

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Georgia Economides*¹, Svenja Falk*¹, Audrey Mercer

¹Department of Pharmacology,University College London

Chapters

00:04Title
00:26Tissue Preparation and Staining for Fluorescence Microscopy and 3D Neuronal Reconstruction
04:08Histological Detection of Biotin-filled Neurons
08:033D Neuronal Reconstructions
10:12Results: Reconstruction of a Basket Cell Recorded and Filled in the Hippocampal CA2 Region and Morphometry Analyses
10:57Conclusion

Summary

Automatic Translation

这里概述的协议描述了免疫荧光分析, 生物细胞素恢复和高质量的重建海马 ca2 神经元后, 细胞内电生理记录在体外, 允许神经元特征, 并最终精细的神经元解剖进行研究。

Tags

Biocytin 3D Reconstruction Hippocampal CA2 Interneurons Neuronal Subtypes Cortical Circuitry Ultrastructure Dendritic Arbor Axonal Arbor Electrophysiology ACSF Fixative Solution Phosphate Buffer Gelatin Vibratome Sucrose

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