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Bioengineering
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3 D ダイナミック文化システムの多層間葉系幹細胞シートの構築
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JoVE Journal
Bioengineering
Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
Please note that all translations are automatically generated.
Click here for the English version.
3 D ダイナミック文化システムの多層間葉系幹細胞シートの構築
DOI:
10.3791/58624-v
•
07:44 min
•
October 20, 2018
•
Yingwei Wang*
1,2
,
Cheng Lu*
1,2
,
Chengzhi He
,
Baoxin Chen
2
,
Youling Zheng
2
,
Junming Zheng
2
,
Jianhua Zhang
,
Zheng Wu
2
1
Key Laboratory for Regenerative Medicine, Ministry of Education
,
Jinan University
,
2
Department of Developmental and Regenerative Biology
,
Jinan University
,
3
Nansha College Preparatory Academy
,
4
Department of Cardiology
,
First Affiliated Hospital of Jinan University
Chapters
00:00
Title
01:02
Preparations Before Cell Sheet Construction
01:39
Preparing Cells for Cell Sheet Construction
03:22
Preparing BMSCs and RAD16-I Peptide Hydrogel Mixture
04:36
In vitro
Culture of 3D Multilayered Cell Sheet Using a Dynamic Culture System
05:18
Obtain the Multilayered MSC Cell Sheet
05:49
Results
06:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
この資料では、プロパティが有利な幹細胞幹細胞の多層シートを構築するため効率的かつ実行可能な方法を提供します。
Tags
Mesenchymal Stem Cell
Cell Sheet
3D Dynamic Culture System
Multilayered Stem Cell Sheet
Pericardium Scaffold
Nonapeptide Hydrogel
Cell Nutrition Supply
Cell Retention
Cell Survival
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