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细胞外通量分析技术在视网膜组织外种中的能量代谢测定
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JoVE Journal Biology
Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis
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细胞外通量分析技术在视网膜组织外种中的能量代谢测定

DOI:
10.3791/58626-v

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10:19 min

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January 07, 2019

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, , , , ,
1Division of Metabolism, Endocrinology and Lipid Research, Department of Medicine,Washington University School of Medicine, 2Department of Biomedical Engineering,Washington University in Saint Louis, 3Department of Ophthalmology and Visual Science,Washington University School of Medicine

Chapters

  • 00:04Title
  • 00:37Calibration of Extracellular Flux Instrument
  • 01:53Isolation of Fresh Mouse Retinal Tissue
  • 03:59Assay Protocol
  • 07:48Results: Energy Metabolism in the Transducin-Null Retina
  • 09:35Conclusion

Summary

Automatic Translation

Automatic Translation

该技术描述了使用细胞外助焊剂分析仪实时记录小鼠视网膜组织的耗氧量和细胞外酸化率。

Tags

Extracellular Flux AnalysisEnergy MetabolismGlycolysisOxidative PhosphorylationMitochondrial StressMouse Retina

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