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Journal
/
Biochemistry
/
低氧环境下人巨噬细胞极化的蛋白质组学分析
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
Please note that all translations are automatically generated.
Click here for the English version.
低氧环境下人巨噬细胞极化的蛋白质组学分析
DOI:
10.3791/58727-v
•
10:15 min
•
January 07, 2019
•
Magali Court
2
,
Marie Malier
2
,
Arnaud Millet
2,3
1
Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309
,
2
Université Grenoble Alpes
,
3
Pôle Recherche
,
Centre Hospitalier Universitaire des Alpes
Chapters
00:04
Title
00:40
Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Leukoreduction System Chamber (LRSC)
02:34
Magnetic Labeling and Isolation of CD14+ Cells (Monocytes)
04:32
Lysis and In-Gel Digestion
07:54
Results: Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
09:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一个协议, 以获得蛋白质组的特征的人类巨噬细胞, 并应用于确定低氧环境对巨噬细胞极化的影响。
Tags
Proteomic Analysis
Human Macrophage Polarization
Low Oxygen Environment
Label-free Quantification
PBMC Isolation
CD14+ Monocyte Isolation
Macrophage Differentiation
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