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Proteomic analyse av menneskelig Macrophage polarisering Under et lavt oksygeninnhold miljø
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
DOI:

10:15 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:40Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Leukoreduction System Chamber (LRSC)
  • 02:34Magnetic Labeling and Isolation of CD14+ Cells (Monocytes)
  • 04:32Lysis and In-Gel Digestion
  • 07:54Results: Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
  • 09:17Conclusion

Summary

Automatic Translation

Vi presenterer en protokoll for å skaffe proteomic signaturer av menneskelig makrofager og dette gjelder fastsettelse av virkningen av et lavt oksygeninnhold miljø macrophage polarisering.

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