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Bioengineering
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三维水凝胶中全球细胞基质金属蛋白酶和代谢活性的测定
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Bioengineering
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JoVE Journal
Bioengineering
Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
Please note that all translations are automatically generated.
Click here for the English version.
三维水凝胶中全球细胞基质金属蛋白酶和代谢活性的测定
DOI:
10.3791/59123-v
•
07:39 min
•
January 22, 2019
•
Abdulaziz S. Fakhouri
2,3
,
Jennifer L. Leight
2
1
Department of Biomedical Engineering
,
The Ohio State University
,
2
The Ohio State University Comprehensive Cancer Center
,
Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
,
3
Biomedical Technology Department
,
King Saud University
Chapters
00:04
Title
00:46
Assay Media and Hydrogel Preparation
01:41
Encapsulate Cells in Hydrogels
03:51
MMP and Metabolic Activity Measurement
05:08
Results: Measuring MMP and Metabolic Activities of Cells Cultured in 3D Fluorogenic Hydrogels
06:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文提出了一种用荧光基质金属蛋白酶 (mmp) 可降解肽对聚 (乙二醇) (peg) 水凝胶进行包封和培养的方案。细胞 mmp 和代谢活性直接从水凝胶培养物测量使用标准的微板读取器。
Tags
3D Hydrogels
Cellular Function
Fluorescent Sensor
Protease Activity
High-throughput Drug Screening
Hydrogel Preparation
Cell Encapsulation
A375 Melanoma Cells
PBS Buffer
96-well Plate
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