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JoVE Journal Bioengineering
Misurazione della proteinasi metallica della matrice cellulare globale e l'attività metabolica in idrogel 3D
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Misurazione della proteinasi metallica della matrice cellulare globale e l'attività metabolica in idrogel 3D

Article DOI: 10.3791/59123-v • 07:39 min • January 22nd, 2019
January 22nd, 2019 •
1,2,3, 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

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Qui, un protocollo è presentato per l'incapsulamento e la coltura delle cellule in poly(ethylene glycol) (PEG) idrogeli funzionalizzati con un fluorogenic metalloproteinasi (MMP)-peptide degradabile. MMP cellulare e dell'attività metabolica sono misurati direttamente dalle culture idrogel utilizzando un lettore di micropiastre standard.

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Bioingegneria numero 143 idrogeli 3D cell incapsulamento metalloproteinasi della matrice attività metabolica Melanoma fluorescenza
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