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JoVE Journal Bioengineering
Mätning av globala Cellular Matrix metalloproteinas och metabolisk aktivitet i 3D Hydrogels
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Mätning av globala Cellular Matrix metalloproteinas och metabolisk aktivitet i 3D Hydrogels

Article DOI: 10.3791/59123-v • 07:39 min • January 22nd, 2019
January 22nd, 2019 •
1,2,3, 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

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Här, ett protokoll presenteras för inkapsling och odla celler i poly(ethylene glycol) (PEG) hydrogels functionalized med en fluorogenic matrix metalloproteinas (MMP)-nedbrytbara peptid. Cellulära MMP och metabolisk aktivitet mäts direkt från hydrogel kulturer använder en standard microplate reader.

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Bioteknik fråga 143 Hydrogels 3D cell inkapsling Matrix metalloproteinaser metabolisk aktivitet melanom fluorescens
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