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JoVE Journal
Developmental Biology

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Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
 
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Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Article doi: 10.3791/59297
April 18th, 2019

Summary April 18th, 2019

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We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.

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