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使用 En 面免疫荧光染色直接观察血管内皮细胞
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Biology
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JoVE Journal
Biology
Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
Please note that all translations are automatically generated.
Click here for the English version.
使用 En 面免疫荧光染色直接观察血管内皮细胞
DOI:
10.3791/59325-v
•
00:06 min
•
August 20, 2019
•
Chang Li*
1,2
,
Zhu Hui Liu*
1,2
,
Jia Wei Chen
2
,
Xin Yi Shu
2
,
Ying Shen
,
Feng Hua Ding
,
Rui Yan Zhang
,
Wei Feng Shen
,
Lin Lu
,
Xiao Qun Wang
1
Department of Cardiology, Ruijin Hospital
,
Shanghai Jiao Tong University School of Medicine
,
2
Institute of Cardiovascular Diseases
,
Shanghai Jiao Tong University
Chapters
00:04
Title
00:35
Perfusion, Dissection, Longitudinally Opening of the Mouse Aorta
02:47
Pretreatment, Immunostaining and Mounting of the Aorta
04:37
Results: Representative En Face Staining Images
05:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出了免疫荧光染色方案,以直接观察小鼠主尾的内皮细胞。该技术在研究不同流动模式的内皮细胞细胞和分子表型以及动脉粥样硬化的发展时非常有用。
Tags
En Face Immunofluorescence
Vascular Endothelial Cells
Fluid Shear Stress
Aorta Dissection
Perfusion
Paraformaldehyde
Permeabilization
Immunostaining
VCAM1
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