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Developmental Biology

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Isolering og farvning af mus hud keratinocytter for celle cyklus specifik analyse af cellulært protein ekspression ved Massecytometri
 
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Isolering og farvning af mus hud keratinocytter for celle cyklus specifik analyse af cellulært protein ekspression ved Massecytometri

Article DOI: 10.3791/59353-v
May 9th, 2019

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Summary May 9th, 2019

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Denne protokol beskriver, hvordan man isolerer hudens keratinocytter fra musemodeller, til farvning med metal mærkede antistoffer, og til at analysere farvede celler ved massecytometri for at profilere udtryks mønstret af proteiner af interesse i de forskellige celle cyklus faser.

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