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Isolering og farvning af mus hud keratinocytter for celle cyklus specifik analyse af cellulært protein ekspression ved Massecytometri
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Chapters
- 00:04Title
- 01:16Preparations
- 02:43Labeling of S phase by IdU Incorporation and Isolation of Cells
- 05:19Cisplatin Labeling and Cell Fixation
- 06:56Barcoding of Samples
- 08:02Labeling of Cells for Mass Cytometry
- 09:48Results: Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
- 11:51Conclusion
Denne protokol beskriver, hvordan man isolerer hudens keratinocytter fra musemodeller, til farvning med metal mærkede antistoffer, og til at analysere farvede celler ved massecytometri for at profilere udtryks mønstret af proteiner af interesse i de forskellige celle cyklus faser.
