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Developmental Biology
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小鼠皮肤角质细胞的分离和染色,用于细胞周期特定分析细胞蛋白表达的量度细胞测定
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
小鼠皮肤角质细胞的分离和染色,用于细胞周期特定分析细胞蛋白表达的量度细胞测定
DOI:
10.3791/59353-v
•
12:34 min
•
May 09, 2019
•
Josiah Fernandez
,
Enrique C Torchia
1
Department of Dermatology and Charles C. Gates Center for Regenerative Medicine
,
University of Colorado Anschutz Medical Campus
Chapters
00:04
Title
01:16
Preparations
02:43
Labeling of S phase by IdU Incorporation and Isolation of Cells
05:19
Cisplatin Labeling and Cell Fixation
06:56
Barcoding of Samples
08:02
Labeling of Cells for Mass Cytometry
09:48
Results: Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
11:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何从小鼠模型中分离皮肤角蛋白细胞,用金属标记的抗体染色,以及通过质量细胞测定法分析染色细胞,以便分析不同细胞周期阶段感兴趣的蛋白质的表达模式。
Tags
Keratinocytes
Cell Cycle
Mass Cytometry
Protein Expression
Cell Proliferation
Cancer Research
Cell Isolation
Skin Cells
IdU
Paraformaldehyde
Cisplatin
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