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血管紧张蛋白II图解的硝酸分析与鉴定
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Nitropeptide Profiling and Identification Illustrated by Angiotensin II
DOI:

07:31 min

June 16, 2019

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Chapters

  • 00:04Title
  • 00:32Nitro-Angiotensin II Desalting
  • 01:30Primary Amine Alkylation by Dimethyl Labeling
  • 02:37Nitrotyrosine to Aminotyrosine Reduction and Biotinylation, Enrichment, and Detection
  • 06:03Results: Representative Mass Spectra Analyses
  • 06:59Conclusion

Summary

Automatic Translation

由于三硝基酪氨酸修饰的丰度较低,酪氨酸硝酸蛋白的蛋白组谱分析一直是一项具有挑战性的技术。在这里,我们描述了一种利用血管紧张蛋白II作为模型来增强和分析硝基肽扩脂的新方法。此方法可以扩展到其他体外体内系统。

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