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Immunology and Infection

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果蝇、果蝇细胞免疫应答的评估

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Immunology and Infection

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JoVE Journal Immunology and Infection

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

Please note that all translations are automatically generated. Click here for the English version.

果蝇、果蝇细胞免疫应答的评估

DOI:

10.3791/59543-v

•

05:50 min

•

April 10, 2019

•

Ashley E. Nazario-Toole², Louisa P. Wu³

¹Department of Biology,University of Maryland, ²Department of Cell Biology and Molecular Genetics,University of Maryland, ³Institute for Bioscience and Biotechnology Research,University of Maryland

Chapters

00:04Title
00:30Fly Injection
02:32Fly Imaging and Fluorescence Quantification and Normalization
03:59Results: Representative In Vivo Phagocytosis Assay Analyses
05:18Conclusion

Summary

Automatic Translation

该方案描述了一种在成虫体内吞噬试验的方法, 以量化吞噬细胞的识别和微生物感染的清除。

Tags

Keywords: Phagocytosis Drosophila Melanogaster In Vivo Assay Immune Response Cellular Immunity Genetic Screen Genome-wide Association Study Pathogen Recognition Pathogen Clearance Trypan Blue Fluorescence Microscopy Imaging Analysis

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