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Journal / Cancer Research / 循环肿瘤细胞的微操作,…
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Cancer Research

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JoVE Journal Cancer Research
循环肿瘤细胞的微操作, 用于下游分子分析和转移电位评估
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循环肿瘤细胞的微操作, 用于下游分子分析和转移电位评估

Article DOI: 10.3791/59677-v • 05:17 min • May 14th, 2019
May 14th, 2019 •
*1, *1, *1, 1,2, 1
1Cancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, 2SIB Swiss Institute of Bioinformatics
* These authors contributed equally

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在这里, 我们提出了一个综合的工作流程, 以确定表型和分子特征的循环肿瘤细胞 (Ctc)。我们将单个和聚集 Ctc 的活体免疫染色和机器人微操作与单细胞技术结合起来, 对转移种子能力进行下游分析和评估。

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癌症研究 第147期 循环肿瘤细胞 循环肿瘤细胞群 微动手术 循环肿瘤细胞培养 单细胞测序 转移 乳腺癌
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