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免疫蛍光による非小細胞肺癌患者からの循環腫瘍細胞の半自動PD-L1特性図と列挙
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
DOI:

10:29 min

August 14, 2019

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Chapters

  • 00:04Title
  • 01:11Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples
  • 03:10Spiral Microfluidic Device CTC Enrichment
  • 04:20Immunofluorescence (IF) Staining
  • 06:32IF Image Acquisition and Analysis
  • 08:07Results: Representative CTC Characterization and Enumeration
  • 09:56Conclusion

Summary

Automatic Translation

循環腫瘍細胞(CTC)の特徴付けは、翻訳研究で一般的な話題です。このプロトコルは、非小細胞肺癌(NSCLC)患者サンプルにおけるCTCのPD-L1特性測定および列挙に関する半自動免疫蛍光(IF)アッセイについて説明する。

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