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免疫荧光对非小细胞肺癌患者循环肿瘤细胞的半自动PD-L1特征化及枚举
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
DOI:

10:29 min

August 14, 2019

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Chapters

  • 00:04Title
  • 01:11Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples
  • 03:10Spiral Microfluidic Device CTC Enrichment
  • 04:20Immunofluorescence (IF) Staining
  • 06:32IF Image Acquisition and Analysis
  • 08:07Results: Representative CTC Characterization and Enumeration
  • 09:56Conclusion

Summary

Automatic Translation

循环肿瘤细胞(CTC)的表征是转化研究的一个热门话题。该协议描述了一种半自动免疫荧光(IF)测定,用于PD-L1表征和非小细胞肺癌(NSCLC)患者样本中CTC的枚举。

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