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免疫荧光对非小细胞肺癌患者循环肿瘤细胞的半自动PD-L1特征化及枚举
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Cancer Research
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JoVE Journal Cancer Research
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
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免疫荧光对非小细胞肺癌患者循环肿瘤细胞的半自动PD-L1特征化及枚举

DOI:
10.3791/59873-v

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10:29 min

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August 14, 2019

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, , , , , , , , , ,
1Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud,Hospices Civils de Lyon, 2Circulating Cancer (CIRCAN) Program,Hospices Civils de Lyon Cancer Institute, 3University of Lyon, Claude Bernard University,Cancer Research Center of Lyon, 4Biolidics Limited, 5Institut de Pathologie Multisites des HCL-Site Sud,Hospices Civils de Lyon, 6Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital,Hospices Civils de Lyon Cancer Institute, 7EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty,Lyon 1 University

Chapters

  • 00:04Title
  • 01:11Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples
  • 03:10Spiral Microfluidic Device CTC Enrichment
  • 04:20Immunofluorescence (IF) Staining
  • 06:32IF Image Acquisition and Analysis
  • 08:07Results: Representative CTC Characterization and Enumeration
  • 09:56Conclusion

Summary

Automatic Translation

Automatic Translation

循环肿瘤细胞(CTC)的表征是转化研究的一个热门话题。该协议描述了一种半自动免疫荧光(IF)测定,用于PD-L1表征和非小细胞肺癌(NSCLC)患者样本中CTC的枚举。

Tags

Circulating Tumor CellsCTCNon-small Cell Lung CancerPD-L1ImmunofluorescenceMicrofluidicCell CharacterizationCell EnrichmentFISHTranscriptomics

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