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Semi-automatisk PD-L1 karakterisering og optælling af cirkulerende tumor celler fra ikke-småcellet lunge cancer patienter ved immunofluorescens
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
DOI:

10:29 min

August 14, 2019

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Chapters

  • 00:04Title
  • 01:11Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples
  • 03:10Spiral Microfluidic Device CTC Enrichment
  • 04:20Immunofluorescence (IF) Staining
  • 06:32IF Image Acquisition and Analysis
  • 08:07Results: Representative CTC Characterization and Enumeration
  • 09:56Conclusion

Summary

Automatic Translation

Karakteriseringen af cirkulerende tumorceller (CTCs) er et populært emne i Translationel forskning. Denne protokol beskriver en semi-automatisk immunofluorescens (IF) assay for PD-L1 karakterisering og optælling af Ctc'er i ikke-småcellet lungekræft (NSCLC) patientprøver.

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