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Semi-automatisk PD-L1 karakterisering og optælling af cirkulerende tumor celler fra ikke-småcellet lunge cancer patienter ved immunofluorescens
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1Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud,Hospices Civils de Lyon, 2Circulating Cancer (CIRCAN) Program,Hospices Civils de Lyon Cancer Institute, 3University of Lyon, Claude Bernard University,Cancer Research Center of Lyon, 4Biolidics Limited, 5Institut de Pathologie Multisites des HCL-Site Sud,Hospices Civils de Lyon, 6Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital,Hospices Civils de Lyon Cancer Institute, 7EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty,Lyon 1 University
Chapters
- 00:04Title
- 01:11Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples
- 03:10Spiral Microfluidic Device CTC Enrichment
- 04:20Immunofluorescence (IF) Staining
- 06:32IF Image Acquisition and Analysis
- 08:07Results: Representative CTC Characterization and Enumeration
- 09:56Conclusion
Karakteriseringen af cirkulerende tumorceller (CTCs) er et populært emne i Translationel forskning. Denne protokol beskriver en semi-automatisk immunofluorescens (IF) assay for PD-L1 karakterisering og optælling af Ctc'er i ikke-småcellet lungekræft (NSCLC) patientprøver.
