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Semi-automatisk PD-L1 karakterisering og opplisting av sirkulerende tumor celler fra ikke-liten celle Lungekreftpasienter ved Immunofluorescence
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
DOI:

10:29 min

August 14, 2019

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Chapters

  • 00:04Title
  • 01:11Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples
  • 03:10Spiral Microfluidic Device CTC Enrichment
  • 04:20Immunofluorescence (IF) Staining
  • 06:32IF Image Acquisition and Analysis
  • 08:07Results: Representative CTC Characterization and Enumeration
  • 09:56Conclusion

Summary

Automatic Translation

Karakterisering av sirkulerende tumorceller (CTC) er et populært tema i translational forskning. Denne protokollen beskriver en semi-automatisk immunofluorescence (IF)-analysen for PD-L1 karakterisering og opplisting av CTC i ikke-liten celle lungekreft (NSCLC) pasientprøver.

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