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Journal / Neuroscience / CRISPR-Cas9を用いたミツバチ脳部における抗RDLおよび抗mGlutR1受容体抗体検査…
JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
CRISPR-Cas9を用いたミツバチ脳部における抗RDLおよび抗mGlutR1受容体抗体検査
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CRISPR-Cas9を用いたミツバチ脳部における抗RDLおよび抗mGlutR1受容体抗体検査

Article DOI: 10.3791/59993-v • 09:25 min • January 30th, 2020
January 30th, 2020 •
1,2, *2, *2, 3, 2, 1, 4, 4, 2
1Department of Neuroscience, University of Arizona, 2School of Life Sciences, Arizona State University, 3Department of Scientific and Technologic Investigations, University of Sonora, 4Integrated DNA Technologies, Inc.
* These authors contributed equally

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ここでは、成人ミツバチの脳におけるタンパク質の産生を減らして抗体特異性をテストするためにCRISPR-Cas9システムを使用するプロトコルです。

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神経科学 問題 155 GABAA受容体 RDL 代謝型グルタミン酸受容体 mGlutR1 共役抗体制御 CRISPR-Cas9
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