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Neuroscience

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JoVE Journal Neuroscience
Enkle synapseindikatorer for glutamatfrigjøring og opptak i akutte hjerneskiver fra normale og huntingtonmus
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Enkle synapseindikatorer for glutamatfrigjøring og opptak i akutte hjerneskiver fra normale og huntingtonmus

Article DOI: 10.3791/60113-v • 08:27 min • March 11th, 2020
March 11th, 2020 •
1, 1
1Synaptic Dysfunction Group, Neuroscience Research Center, Charité - University Medicine

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Vi presenterer en protokoll for å evaluere balansen mellom glutamat frigjøring og klaring ved enkelt kortikostriatal glutamatergic synapser i akutte skiver fra voksne mus. Denne protokollen bruker fluorescerende sensor iGluu for glutamat deteksjon, et sCMOS kamera for signaloppkjøp og en enhet for fokal laser belysning.

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Nevrovitenskap glutamat frigjøring glutamat clearance glutamat spredning presynaptisk terminal astrocytt glutamat opptak forfall kinetikk spill-over optogenetikk iGluu sCMOS
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