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Neuroscience
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正常小鼠和亨廷顿小鼠急性脑切片中谷氨酸释放和服用的单突触指标
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
Please note that all translations are automatically generated.
Click here for the English version.
正常小鼠和亨廷顿小鼠急性脑切片中谷氨酸释放和服用的单突触指标
DOI:
10.3791/60113-v
•
08:27 min
•
March 11, 2020
•
Anton Dvorzhak
,
Rosemarie Grantyn
1
Synaptic Dysfunction Group, Neuroscience Research Center
,
Charité – University Medicine
Chapters
00:04
Introduction
00:28
Autofluorescence Correction
01:48
Bouton Search
03:19
Glutamate Release and Clearance Visualization
04:49
Off-Line Glutamate Transient Construction and Rapid Glutamate Release and Clearance Assessment
06:01
Results: Representative Identification of Dysfunctional Synapses in Q175 Mice with a Hypokinetic Phenotype
07:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一个协议,以评估成年小鼠急性切片中单皮质谷胱甘肽突触的谷氨酸释放和间隙之间的平衡。该协议使用荧光传感器iGlu
u
进行谷氨酸检测,sCMOS摄像机用于信号采集和用于聚焦激光照明的设备。
Tags
Single Synapse
Glutamate Release
Glutamate Uptake
Acute Brain Slices
Huntington Mice
One-photon Microscope
Autofluorescence Correction
Dorsal Striatum
Stimulation Pipette
Action Potential Dependent Release
Ion Channel Blockers
Membrane Receptors
Tetrototoxin
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