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Bioengineering
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在微流体进化加速器上生成跨癌症人群的异构药物梯度,用于实时观察
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Bioengineering
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JoVE Journal
Bioengineering
Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation
Please note that all translations are automatically generated.
Click here for the English version.
在微流体进化加速器上生成跨癌症人群的异构药物梯度,用于实时观察
DOI:
10.3791/60185-v
•
10:24 min
•
September 19, 2019
•
Ke-Chih Lin
,
Gonzalo Torga
,
Yusha Sun
,
Kenneth J. Pienta
,
James C. Sturm
,
Robert H. Austin
1
Princeton University
,
2
Johns Hopkins Medical Institute
Chapters
00:04
Title
01:09
Fabrication of Metal Plate Holder and Cell Line Seeding
03:05
Setting up Thermal Control Unit and Gas Supply System
03:50
Installation of Microfluidic Device
08:12
Results: Growth Curves and Cell Motility Assay
09:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个微流体癌片模型,即"进化加速器"技术,它为在单细胞明确定义的环境条件下癌症动力学的长期实时定量研究提供了一个可控的平台水平。这项技术有望成为基础研究或临床前药物开发的体外模型。
Tags
Microfluidic Cancer Model
Evolution Accelerator
Real-time Observation
Heterogeneous Drug Gradients
Tumor-like Stress Landscape
Preclinical Drug Development
Pressure Sealing Packaging
Long-term Experiment
Gas Composition
Cell Line Seeding
PC3-Epi
PC3-EMT
PDMS Devices
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