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大鼠中枢神经系统中反义寡核苷酸的细胞内传递
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Neuroscience
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JoVE Journal Neuroscience
Intrathecal Delivery of Antisense Oligonucleotides in the Rat Central Nervous System
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大鼠中枢神经系统中反义寡核苷酸的细胞内传递

DOI:
10.3791/60274-v

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07:47 min

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October 29, 2019

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Chapters

  • 00:04Title
  • 00:46Material and Instrument Preparation
  • 02:09Surgery Preparation
  • 03:05Surgery
  • 06:53Results: A Single Bolus Antisense Oligonucleotide (ASO) Intrathecal Injection Reduces Rat Malat1 In Vivo
  • 07:17Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一种通过将导管植入脊柱的腰间空间来向大鼠中枢神经系统输送药物的方法。我们专注于反义寡核苷酸的输送，尽管这种方法也适合其他治疗方式的交付。

Tags

Intrathecal DeliveryAntisense OligonucleotidesRat Central Nervous SystemPharmacokineticsPharmacodynamicsEfficacyGuide CannulaCatheter Wire AssemblyDelivery Catheter AssemblyGuide Cannula-needle AssemblySpinal Cord InjectionBuprenorphineSurgical Procedure

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