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Neuroscience
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5つの脊椎動物群にわたる中枢神経系微小血管の信頼性の高い絶縁
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JoVE Journal
Neuroscience
Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups
Please note that all translations are automatically generated.
Click here for the English version.
5つの脊椎動物群にわたる中枢神経系微小血管の信頼性の高い絶縁
DOI:
10.3791/60291-v
•
10:35 min
•
January 12, 2020
•
Yinyu Yuan*
1
,
Jacquelyn R. Dayton*
1
,
Marie-Lena Freese
,
Bryce G. Dorflinger
,
Lillian Cruz-Orengo
1
Department of Anatomy, Physiology and Cell Biology
,
University of California Davis
,
2
University of Veterinary Medicine Hannover Foundation
Chapters
00:04
Title
00:40
Central Nervous System (CNS) Tissue Dissection
02:35
CNS Tissue Homogenization
04:20
Microvessel Purification
05:57
Microvessel Elution and Filtration
07:44
Results: Representative Microvessel Protein Expression
09:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルの目的は、リッセンセファリックおよびギレンセファリック脊椎動物の中枢神経系の複数の領域から微小血管を分離することです。
Tags
Microvessel Isolation
Central Nervous System
Vertebrates
Dissection
Meninges
Choroid Plexus
Homogenization
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