Journal
/
/
Pneumococcus infektion af primære humane endotelceller i konstant flow
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
DOI:

09:34 min

October 31, 2019

, , ,

Chapters

  • 00:04Title
  • 01:29Endothelial Cell Cultivation of HUVEC, Induction of VWF (Von Willebrand Factor)-release and Visualization of Multimerized VWF Strings
  • 05:53Microscopic Evaluation of Bacterial Attachment to VWF-strings in Flow in Real Time and after Sample Fixation
  • 07:12Results: Analyses of Bacterial Attachment to VWF Strings
  • 08:33Conclusion

Summary

Automatic Translation

Denne undersøgelse beskriver den mikroskopiske monitorering af pneumokokker-tilslutningen til von Willebrand-faktor strenge, der produceres på overfladen af differentierede humane primære endotelceller under forskydnings stress i definerede strømningsforhold. Denne protokol kan udvides til detaljeret visualisering af specifikke cellestrukturer og kvantificering af bakterier ved anvendelse af differentiale immunofarvnings procedurer.

Related Videos

Read Article