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Pneumococcus infektion af primære humane endotelceller i konstant flow
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1Institut für Mikrobiologie,Technische Universität Braunschweig, 2Devision of Infection Medicine, Department of Clinical Science,Lund University, 3Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute,Ludwig Maximilians University, 4Department of Molecular Infection Biology,Helmholtz Center for Infection Research
Chapters
- 00:04Title
- 01:29Endothelial Cell Cultivation of HUVEC, Induction of VWF (Von Willebrand Factor)-release and Visualization of Multimerized VWF Strings
- 05:53Microscopic Evaluation of Bacterial Attachment to VWF-strings in Flow in Real Time and after Sample Fixation
- 07:12Results: Analyses of Bacterial Attachment to VWF Strings
- 08:33Conclusion
Denne undersøgelse beskriver den mikroskopiske monitorering af pneumokokker-tilslutningen til von Willebrand-faktor strenge, der produceres på overfladen af differentierede humane primære endotelceller under forskydnings stress i definerede strømningsforhold. Denne protokol kan udvides til detaljeret visualisering af specifikke cellestrukturer og kvantificering af bakterier ved anvendelse af differentiale immunofarvnings procedurer.
