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Generation af Ortotopiske tumorer i bugspytkirtlen og ex vivo karakterisering af tumor Infiltrerende T-celle cytotoksicitet
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity
DOI:

06:16 min

December 07, 2019

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Chapters

  • 00:04Title
  • 00:37Digestion of Pancreatic Tumors
  • 01:24Preparation of Single-cell Suspension from Digested Tumor
  • 03:04Preparing Cells for Ex Vivo Stimulation
  • 03:57Results: Flow Cytometry Analysis and Quantification of Ex Vivo Spleen- and Tumor-derived T Cell Activity
  • 05:29Conclusion

Summary

Automatic Translation

Denne protokol beskriver den kirurgiske generation af ortotopiske pancreastumorer og den hurtige fordøjelse af frisk isolerede murine pancreas tumorer. Efter fordøjelsen kan levedygtige immuncelle populationer anvendes til yderligere downstream-analyse, herunder ex vivo -stimulering af T-celler til intracellulær cytokindetektion ved strømnings cytometri.

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