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基于无标签阻抗的 GPCR 检测中用于提高吞吐量的逐步加量协议
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
DOI:

06:13 min

February 21, 2020

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Chapters

  • 00:04Introduction
  • 01:01Electrode Array Cell Seeding and Equilibration
  • 01:41Cell Equilibration Monitoring and Serial Agonist Addition
  • 03:06Data Export and Analysis
  • 03:52Results: Representative Impedance-Based GPCR Analyses
  • 05:15Conclusion

Summary

Automatic Translation

该协议演示了使用无标签阻抗测量从单个微电极上生长的单个细胞层的激动剂诱导GPCR活化的全剂量-反应关系的实时记录。新的配量方案显著提高了吞吐量,而不会损失时间分辨率。

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