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基于无标签阻抗的 GPCR 检测中用于提高吞吐量的逐步加量协议
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Biochemistry
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JoVE Journal Biochemistry
Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
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基于无标签阻抗的 GPCR 检测中用于提高吞吐量的逐步加量协议

DOI:
10.3791/60686-v

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06:13 min

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February 21, 2020

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, , , , , , , ,
1Institute of Analytical Chemistry, Chemo- and Biosensors,University of Regensburg, 2Institute of Pharmacy,University of Regensburg, 3Department of Chemistry and Pharmacy,Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

Chapters

  • 00:04Introduction
  • 01:01Electrode Array Cell Seeding and Equilibration
  • 01:41Cell Equilibration Monitoring and Serial Agonist Addition
  • 03:06Data Export and Analysis
  • 03:52Results: Representative Impedance-Based GPCR Analyses
  • 05:15Conclusion

Summary

Automatic Translation

Automatic Translation

该协议演示了使用无标签阻抗测量从单个微电极上生长的单个细胞层的激动剂诱导GPCR活化的全剂量-反应关系的实时记录。新的配量方案显著提高了吞吐量，而不会损失时间分辨率。

Tags

Stepwise DosingLabel-free ImpedanceGPCR AssaysThroughputSingle TransductionOrgan-on-chipCell SeedingImpedance RecordingAgonist ModeSerial Addition

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