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使用亲脂荧光染料的果蝇胚胎运动神经元的逆行追踪
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Developmental Biology
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JoVE Journal Developmental Biology
Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
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使用亲脂荧光染料的果蝇胚胎运动神经元的逆行追踪

DOI:
10.3791/60716-v

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08:25 min

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January 12, 2020

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, ,
1Department of Cellular Biology,University of Georgia

Chapters

  • 00:04Title
  • 00:49Preparation of Equipment
  • 02:03Embryo Staging
  • 03:10Dissection and Staining
  • 04:14Dye Injection and Confocal Microscopy
  • 06:45Results: Retrograde Labeling of aCC and RP3 Motor Neurons
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种使用亲脂荧光染料逆行追踪果蝇胚胎运动神经元的方法。

Tags

DrosophilaEmbryonicMotor NeuronsLipophilicFluorescent DyesRetrograde TracingNeuronal MorphogenesisConnectivityAxon TerminalMicroinjectorDissectionEmbryo CollectingFiltrationMicropipetteBevelingBleachDechorionation

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