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Flow cytometrisk analyse af lymfocyt infiltration i centralnervesystemet under eksperimentel autoimmun encephalomyelitis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

Flow cytometrisk analyse af lymfocyt infiltration i centralnervesystemet under eksperimentel autoimmun encephalomyelitis

DOI:
10.3791/61050-v

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09:01 min

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November 17, 2020

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, , , ,
1Translational Medicine Research Center, Ruijin Hospital North,Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology,Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation,Shanghai Jiao Tong University School of Medicine

Chapters

  • 00:04Introduction
  • 00:53C57BL/6 Mouse Immunization
  • 01:58Single Brain Cell Suspension Preparation
  • 04:19Flow Cytometric Analysis
  • 07:00Results: Representative Experimental Autoimmune Encephalomyelitis (EAE) Course and Brain Lymphocyte Analyses
  • 08:22Conclusion

Summary

Automatic Translation

Automatic Translation

Dette manuskript præsenterer en protokol til at fremkalde aktiv eksperimentel autoimmun encephalomyelitis (EAE) i mus. En metode til isolering og karakterisering af de infiltrerede lymfocytter i centralnervesystemet (CNS) præsenteres også for at vise, hvordan lymfocytter er involveret i udviklingen af CNS autoimmun sygdom.

Tags

Keywords: Flow CytometryLymphocyte InfiltrationCentral Nervous SystemExperimental Autoimmune EncephalomyelitisImmune CellsMOG 35-55Pertussis ToxinNeurological Signs

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