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Immunology and Infection
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实验自身免疫性脑脊髓炎中枢神经系统淋巴细胞渗透的流量细胞计量分析
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
Please note that all translations are automatically generated.
Click here for the English version.
实验自身免疫性脑脊髓炎中枢神经系统淋巴细胞渗透的流量细胞计量分析
DOI:
10.3791/61050-v
•
09:01 min
•
November 17, 2020
•
Zhe Ji
2
,
Chenghua Zhou
,
Hongshen Niu
,
Jinshen Wang
,
Lei Shen
1
Translational Medicine Research Center, Ruijin Hospital North
,
Shanghai Jiao Tong University School of Medicine
,
2
Department of Microbiology and Immunology
,
Tongji University School of Medicine
,
3
Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation
,
Shanghai Jiao Tong University School of Medicine
Chapters
00:04
Introduction
00:53
C57BL/6 Mouse Immunization
01:58
Single Brain Cell Suspension Preparation
04:19
Flow Cytometric Analysis
07:00
Results: Representative Experimental Autoimmune Encephalomyelitis (EAE) Course and Brain Lymphocyte Analyses
08:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本手稿提供了在小鼠中诱导活性实验性自身免疫性脑脊髓炎 (EAE) 的协议。还提出了一种分离和表征中枢神经系统(CNS)中渗透淋巴细胞的方法,以表明淋巴细胞如何参与中枢自身免疫性疾病的发展。
Tags
Flow Cytometry
Lymphocyte Infiltration
Central Nervous System
Experimental Autoimmune Encephalomyelitis
Immune Cells
MOG 35-55
Pertussis Toxin
Neurological Signs
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