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August 27, 2020
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Burada, konfokal mikroskopi için uygun ve aynı zamanda tekrarlanabilir ve güvenilir etiketlemeye izin vermek için Drosophila testislerinin tüm montaj hazırlığı ve immünostainingi için geliştirilmiş bir protokol sunuyoruz. Bu protokolü, büyük S4-S5 spermatositleri üzerindeki kolokalizasyon deneylerinin 3D gösterimi ve niceliği ile göstermektedir.
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Cite this Article
Raich, N., Contremoulins, V., Karess, R. E. Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes. J. Vis. Exp. (162), e61061, doi:10.3791/61061 (2020).
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