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使用纳米流体技术的单体和散装 C. 电子样品 中的高通量定量 RT-PCR
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
DOI:

08:19 min

May 28, 2020

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Chapters

  • 00:05Introduction
  • 00:46Worm-to-CT Worm Lysis
  • 02:08Reverse Transcription (RT) for Bulk Samples
  • 02:38Target Specific Preamplification
  • 03:19Exonuclease I Treatment
  • 03:53Sample Mix Preparation
  • 04:48Nanofluidic Chip Priming and Loading
  • 06:00Results: Representative Worm-to-CT Analysis
  • 07:45Conclusion

Summary

Automatic Translation

本文描述了一个高通量协议,用于快速、可靠地确定单个或批量 C.elegans 样本中的基因表达水平。此协议不需要 RNA 隔离,并且直接从样品中生成 cDNA。它可以与高通量多路复用纳米流体实时 qPCR 平台一起使用。

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