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Isolering af centralnervesystemvæv og tilhørende meninges til downstream-analyse af immunceller
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
DOI:

09:35 min

May 19, 2020

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Chapters

  • 00:05Introduction
  • 00:53Processing Brain and Spinal Cord Samples
  • 02:01Preparation of the Meninges and CNS Tissues for Flow Cytometry Staining
  • 04:33Preparation of Single‐Cell Suspensions of Brain and Spinal Cord Tissue
  • 06:57Results: Immune Cells in CNS Tissues of TMEV‐IDD Mice
  • 08:50Conclusion

Summary

Automatic Translation

Dette papir præsenterer to optimerede protokoller til undersøgelse af residente og perifert afledte immunceller i centralnervesystemet, herunder hjernen, rygmarven og meninges. Hver af disse protokoller hjælper med at fastslå funktionen og sammensætningen af cellerne, der optager disse rum under steady state og inflammatoriske tilstande.

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