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Immunology and Infection

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分离中枢神经系统组织和相关脑膜，用于免疫细胞的下游分析

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Immunology and Infection

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JoVE Journal Immunology and Infection

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

Please note that all translations are automatically generated. Click here for the English version.

分离中枢神经系统组织和相关脑膜，用于免疫细胞的下游分析

DOI:

10.3791/61166-v

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09:35 min

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May 19, 2020

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Krista D. DiSano, Michael R. Linzey², Nora C. Welsh², Joshua S. Meier, Andrew R. Pachner, Francesca Gilli

¹Department of Neurology, Geisel School of Medicine,Dartmouth-Hitchcock Medical Center, ²Program in Experimental and Molecular Medicine,Dartmouth College

Chapters

00:05Introduction
00:53Processing Brain and Spinal Cord Samples
02:01Preparation of the Meninges and CNS Tissues for Flow Cytometry Staining
04:33Preparation of Single‐Cell Suspensions of Brain and Spinal Cord Tissue
06:57Results: Immune Cells in CNS Tissues of TMEV‐IDD Mice
08:50Conclusion

Summary

Automatic Translation

本文提出了两种优化的方案，用于检查中枢神经系统内的常驻和外周来源的免疫细胞，包括大脑、脊髓和脑膜。这些协议中的每一个都有助于确定在稳态和炎症条件下占据这些隔室的细胞的功能和组成。

Tags

Meninges Immune Cells Single Cell Analysis Histological Methods Microglia Astrocytes Pericytes Endothelial Cells Stromal Cells Neurons Brain Spinal Cord Decalcification Skull Cap Vertebrae Column

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