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09:35 min
May 19, 2020
DOI:
10.3791/61166-v
本文提出了两种优化的方案,用于检查中枢神经系统内的常驻和外周来源的免疫细胞,包括大脑、脊髓和脑膜。这些协议中的每一个都有助于确定在稳态和炎症条件下占据这些隔室的细胞的功能和组成。
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Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
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Cite this Article
DiSano, K. D., Linzey, M. R., Welsh, N. C., Meier, J. S., Pachner, A. R., Gilli, F. Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells. J. Vis. Exp. (159), e61166, doi:10.3791/61166 (2020).
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