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肺环境中免疫细胞和促炎介质的表征
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
DOI:

09:00 min

June 24, 2020

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Chapters

  • 00:04Introduction
  • 00:45Lung Tissue Harvest
  • 02:50Lung Tissue Homogenization
  • 03:44Lung Tissue Dissociation and Single Cell Isolation
  • 05:21Flow Cytometric Lung Immune Cell Niche Analysis
  • 06:27Results: Representative Immune Cell Population and Chemokine Expression Characterization
  • 08:35Conclusion

Summary

Automatic Translation

该协议描述了使用流式细胞术来鉴定短暂性脑中动脉闭塞后肺部环境中免疫细胞组成,细胞因子谱和趋化因子谱的变化,这是缺血性中风的小鼠模型。

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