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Immunology and Infection

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Caratterizzazione di cellule immunitarie e mediatori proinfiammatori in ambiente polmonare

Article DOI: 10.3791/61359-v • 09:00 min
June 24th, 2020 •
*1, *1, 1, 1,2,3
1Department of Microbiology, Immunology, and Cell Biology, West Virginia University, 2Department of Neuroscience, West Virginia University, 3Rockefeller Neuroscience Institute, West Virginia University
* These authors contributed equally

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June 24th, 2020

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Questo protocollo descrive l'uso della citometria a flusso per identificare i cambiamenti nella composizione delle cellule immunitarie, nel profilo delle citochine e nel profilo delle chemochine nell'ambiente polmonare dopo occlusione transitoria dell'arteria cerebrale media, un modello murino di ictus ischemico.

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