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Karakterisering av immunceller og proinflammatoriske mediatorer i lungemiljøet
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
DOI:

09:00 min

June 24, 2020

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Chapters

  • 00:04Introduction
  • 00:45Lung Tissue Harvest
  • 02:50Lung Tissue Homogenization
  • 03:44Lung Tissue Dissociation and Single Cell Isolation
  • 05:21Flow Cytometric Lung Immune Cell Niche Analysis
  • 06:27Results: Representative Immune Cell Population and Chemokine Expression Characterization
  • 08:35Conclusion

Summary

Automatic Translation

Denne protokollen beskriver bruken av flowcytometri for å identifisere endringene i immuncellesammensetning, cytokinprofil og kjemokinprofil i lungemiljøet etter forbigående okklusjon av cerebri cerebralis, en murine modell av iskemisk hjerneslag.

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