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Immunology and Infection
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活细胞向前遗传方法,识别和分离衣
原体沙眼中的发育突变体
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in
Chlamydia trachomatis
Please note that all translations are automatically generated.
Click here for the English version.
活细胞向前遗传方法,识别和分离衣
原体沙眼中的发育突变体
DOI:
10.3791/61365-v
•
10:32 min
•
June 10, 2020
•
Travis J. Chiarelli
,
Nicole A. Grieshaber
,
Scott S. Grieshaber
1
Department of Biological Sciences
,
University of Idaho
Chapters
00:04
Introduction
00:30
Chlamydia trachomatis
(Ctr) Reporter Mutagenesis
01:34
Mutant Ctr Imaging
03:09
Microscope Setup and Imaging
04:25
Altered Developmental Mutagenized Chlamydia Phenotype Identification and Isolation
07:07
Mutant Isolate Phenotype Verification
08:06
Results: Representative Ctr Developmental Mutant Identification
09:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议利用荧光启动子-记者,活细胞显微镜,和个人包含提取在定向前的基因方法,以识别和分离
衣原体沙眼的发育突变体
。
Tags
Chlamydia Trachomatis
Live-cell
Forward Genetic Approach
Developmental Mutants
Reporter
Mutagenesis
EMS
Host Cell Culture
Infection
Isolation
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