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胎儿接触产妇炎症的穆林模型,研究急性胆管炎对新生儿肠道发育的影响
JoVE Journal
Medicine
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Medicine
A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development
Please note that all translations are automatically generated.
Click here for the English version.
胎儿接触产妇炎症的穆林模型,研究急性胆管炎对新生儿肠道发育的影响
DOI:
10.3791/61464-v
•
08:50 min
•
June 24, 2020
•
Brian A. Juber
,
Timothy G. Elgin
,
Erin M. Fricke
,
Huyiu Gong
,
Jeffrey Reese
,
Steven J. McElroy
4
1
Division of Neonatology, Stead Family Department of Pediatrics
,
University of Iowa
,
2
Division of Maternal Fetal Medicine, Department of Obstetrics & Gynecology
,
University of Iowa
,
3
Division of Neonatology, Department of Pediatrics
,
Vanderbilt University
,
4
Department of Microbiology & Immunology
,
University of Iowa
Chapters
00:04
Introduction
00:52
Establishment of FEMI in Pregnant Mice
02:06
Delivery and Care of Offspring and Intestinal Harvesting
03:03
Intestinal Injury Scoring
04:11
Quantification of Paneth and Goblet Cells
06:35
Results: Intestinal Injury of Newborn Mice Induced by FEMI
08:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们开发了一个胆管炎模型,以模拟胎儿接触产妇炎症(FEMI),而不会引起活体并发症,以检查FEMI对后代肠道发育的影响。这允许研究胆管炎后肠道损伤发展的机械原因。
Tags
Fetal Exposure
Maternal Inflammation
FEMI
Necrotizing Enterocolitis
NEC
Chorioamnionitis
LPS Injection
Pregnant Mouse
Intestinal Development
RNA Quantification
Histology
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