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Journal
/
Medicine
/
ラットにおける骨髄間葉系幹細胞の単離と培養
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Medicine
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JoVE Journal
Medicine
Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats
Please note that all translations are automatically generated.
Click here for the English version.
ラットにおける骨髄間葉系幹細胞の単離と培養
DOI:
10.3791/61532-v
•
08:58 min
•
August 25, 2020
•
Yueyang Hong*
1
,
Hongyuan Xu*
1
,
Yiling Yang
,
Siru Zhou
,
Anting Jin
,
Xiangru Huang
,
Qinggang Dai
,
Lingyong Jiang
1
Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
,
National Clinical Research center of Stomatology
,
2
The 2nd Dental Center, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
,
National Clinical Research center of Stomatology
Chapters
00:05
Introduction
01:10
Harvesting Rat Mandibles
02:21
Isolation and Cultivation of the mBMSCs
03:39
Flow Cell Sorting
06:24
Results: Osteogenic, Adipogenic, and Chondrogenic Differentiation of mBMSCs
08:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
本稿では、ラット下顎骨から骨髄間葉幹細胞を単離、栽培、選別、同定するための骨髄付着とフローサイトメトリーの選別を組み合わせた方法を紹介する。
Tags
Mandibular
Bone Marrow
Mesenchymal Stem Cells
Isolation
Cultivation
Rats
Cranial Facial Bone
BMSCs
Bone Marrow Adherence
Fluorescent Cell Sorting
Mandibular Body
Mandibular Ramus
Coracoid
Condyle
Marrow Cavity
Alpha-MEM
FBS
Centrifuge
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