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Journal
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Immunology and Infection
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小鼠B细胞发育的流式细胞学表征
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Flow Cytometric Characterization of Murine B Cell Development
Please note that all translations are automatically generated.
Click here for the English version.
小鼠B细胞发育的流式细胞学表征
DOI:
10.3791/61565-v
•
08:25 min
•
January 22, 2021
•
Faith M. Harris*
1
,
Karoline A. Meagher*
1
,
Maggie Zhong
,
Benjamin J. Daniel
,
Mark Eckersdorff
,
Jesse A. Green
,
Vera Voronina
,
Chunguang Guo
,
Andre Limnander
,
Lynn E. Macdonald
1
Regeneron Tech Centers
,
Regeneron Pharmaceuticals, Inc.
,
2
Regeneron Therapeutic Proteins
,
Regeneron Pharmaceuticals, Inc.
,
3
Immunology and Inflammation
,
Regeneron Pharmaceuticals, Inc.
Chapters
00:04
Introduction
00:55
Stain Cells and Prepare Compensation
05:30
Flow Cytometric Data Acquisition and Analysis
06:08
Results: Flow Cytometric Analysis for the Characterization of B Cells
07:24
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们在这里描述了通过流式细胞术对腹膜,脾脏和骨髓组织中小鼠免疫B细胞室异质性的简单分析。该协议可以适应并扩展到其他小鼠组织。
Tags
Flow Cytometry
B Cell Development
Murine Models
Autoimmunity
Antibody Therapeutics
Fluorophores
Phenotyping
Genetic Manipulation
Bone Marrow
Spleen
Viability Dye
Single Color Compensation
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