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小鼠B细胞发育的流式细胞学表征
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometric Characterization of Murine B Cell Development
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小鼠B细胞发育的流式细胞学表征

DOI:
10.3791/61565-v

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08:25 min

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January 22, 2021

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, , , , , , , , ,
1Regeneron Tech Centers,Regeneron Pharmaceuticals, Inc., 2Regeneron Therapeutic Proteins,Regeneron Pharmaceuticals, Inc., 3Immunology and Inflammation,Regeneron Pharmaceuticals, Inc.

Chapters

  • 00:04Introduction
  • 00:55Stain Cells and Prepare Compensation
  • 05:30Flow Cytometric Data Acquisition and Analysis
  • 06:08Results: Flow Cytometric Analysis for the Characterization of B Cells
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

我们在这里描述了通过流式细胞术对腹膜，脾脏和骨髓组织中小鼠免疫B细胞室异质性的简单分析。该协议可以适应并扩展到其他小鼠组织。

Tags

Flow CytometryB Cell DevelopmentMurine ModelsAutoimmunityAntibody TherapeuticsFluorophoresPhenotypingGenetic ManipulationBone MarrowSpleenViability DyeSingle Color Compensation

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