JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
活细菌中蛋白质-蛋白质相互作用的FLIM-FRET测量。
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
Please note that all translations are automatically generated.
Click here for the English version.
活细菌中蛋白质-蛋白质相互作用的FLIM-FRET测量。
DOI:
10.3791/61602-v
•
09:26 min
•
August 25, 2020
•
Hanna Manko
,
Vincent Normant
3
,
Quentin Perraud
3
,
Tania Steffan
,
Véronique Gasser
3
,
Emmanuel Boutant
,
Éléonore Réal
,
Isabelle J. Schalk
3
,
Yves Mély
,
Julien Godet
4
1
Université de Strasbourg
,
Laboratoire de Bioimagerie et Pathologies, UMR CNRS 7021
,
2
Université de Strasbourg, UMR 7242, ESBS
,
3
CNRS, UMR 7242, ESBS
,
4
Groupe Méthode Recherche Clinique
,
Hôpitaux Universitaires de Strasbourg
Chapters
00:04
Introduction
00:41
Fluorescent Tag Insertion into the Chromosomal Genome of
P. aeruginosa
03:02
Preparation of Agarose Pad
03:57
Imaging with a Two-photon Microscopy Setup
06:44
Results: FLIM-FRET Data Visualization and Analysis
08:57
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们在此描述一个协议,使用FLIM-FRET测量来描述活
伪多
西诺萨中两种高度不同的表达蛋白之间的蛋白质-蛋白质相互作用。该协议包括细菌菌株结构、细菌固定、成像和成像后数据分析程序。
Tags
FLIM-FRET
Protein-protein Interactions
Live Bacteria
Live Cells
P.aeruginosa
E.coli
Fluorescent Proteins
Microscopy
Agarose
Gentamycin
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
基于分体式泛素膜酵母双杂交(神话)系统:一个强大的工具用于识别蛋白质 - 蛋白质相互作用
微流体调查可溶性信号介导的相互作用的上皮细胞和细菌的共同文化
微图案的表面上的蛋白质-蛋白质相互作用
的
体内检测
成像蛋白质 - 蛋白质相互作用
在体内</em
蛋白质 - 蛋白质相互作用的双分子荧光互补的烟草原生质体和叶片可视化
研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移
在Cytokineticž圈的活细菌超分辨率成像使用快速三维结构照明显微镜(F3D-SIM卡)
探讨高密度功能蛋白微阵列来检测蛋白质 - 蛋白质相互作用
膜蛋白的实时测量:受体相互作用使用表面等离子体共振(SPR)
的蛋白质 - 蛋白质相互作用抑制剂的发展,通过替换:应用程序的设计和开发非ATP竞争性CDK抑制剂
Read Article