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使用自由浮动组织切片进行基于组织学的染色
JoVE Journal
Biology
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JoVE Journal Biology
Histological-Based Stainings Using Free-Floating Tissue Sections
DOI:

06:45 min

August 25, 2020

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Chapters

  • 00:05Introduction
  • 00:45Cryosectioning and Tissue Section Storage
  • 01:39Staining: Day 1
  • 02:55Staining: Day 2
  • 03:57Mounting
  • 05:09Results: Representative Immunohistochemical Analysis of Free-Floating Tissue Sections
  • 06:02Conclusion

Summary

Automatic Translation

自由浮动技术允许研究人员进行基于组织学的染色,包括对固定组织切片进行免疫组织化学,以可视化生物结构、细胞类型以及蛋白质表达和定位。这是一种高效可靠的组织化学技术,可用于研究多种组织,例如大脑、心脏和肝脏。

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