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小鼠河马兴奋和类特异性抑制神经元的不同组织型切片培养的单细胞电位

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

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小鼠河马兴奋和类特异性抑制神经元的不同组织型切片培养的单细胞电位

DOI:

10.3791/61662-v

•

06:21 min

•

October 06, 2020

•

David G. Keener*^1,2, Amy Cheung*^1,3, Kensuke Futai

¹Brudnick Neuropsychiatric Research Institute, Department of Neurobiology,University of Massachusetts Medical School, ²Interdisciplinary Graduate Program,University of Massachusetts Medical School, ³UMMS MD/PhD Program,University of Massachusetts Medical School

Chapters

00:04Introduction
00:46Electroporation Preparation
03:03Electroporation
04:58Results: Representative Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neuron Electroporation Efficiency
05:45Conclusion

Summary

Automatic Translation

这里介绍的是一个单细胞电位化协议，可以在一系列体外海马切片培养年龄内提供兴奋和抑制神经元的基因。我们的方法提供精确和高效的基因表达在单个细胞，可用于检查细胞自主和细胞间功能。

Tags

Single-cell Electroporation Organotypic Slice Culture Mouse Hippocampus Excitatory Neurons Inhibitory Neurons Gene Transfection Neurobiology Electroporation Protocol High Transfection Efficiency Cell-autonomous Mechanisms Transsynaptic Protein Interactions Slice Culture Preparation Electroporator Setup Pipette Preparation Slice Culture Isolation Target Cell Electroporation

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