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Single-Cell Electroporation på tværs af forskellige organotypiske Slice Kultur Af Mouse Hippocampal Excitatory og klasse-specifikke hæmmende neuroner
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Neuroscience
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JoVE Journal Neuroscience
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

Single-Cell Electroporation på tværs af forskellige organotypiske Slice Kultur Af Mouse Hippocampal Excitatory og klasse-specifikke hæmmende neuroner

DOI:
10.3791/61662-v

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06:21 min

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October 06, 2020

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, ,
1Brudnick Neuropsychiatric Research Institute, Department of Neurobiology,University of Massachusetts Medical School, 2Interdisciplinary Graduate Program,University of Massachusetts Medical School, 3UMMS MD/PhD Program,University of Massachusetts Medical School

Chapters

  • 00:04Introduction
  • 00:46Electroporation Preparation
  • 03:03Electroporation
  • 04:58Results: Representative Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neuron Electroporation Efficiency
  • 05:45Conclusion

Summary

Automatic Translation

Automatic Translation

Præsenteret her er en protokol for enkelt-celle elektroporation, der kan levere gener i både excitatoriske og hæmmende neuroner på tværs af en række in vitro hippocampal skive kultur aldre. Vores tilgang giver præcis og effektiv ekspression af gener i individuelle celler, som kan bruges til at undersøge celle-autonome og intercellulære funktioner.

Tags

Single-cell ElectroporationOrganotypic Slice CultureMouse HippocampusExcitatory NeuronsInhibitory NeuronsGene TransfectionNeurobiologyElectroporation ProtocolHigh Transfection EfficiencyCell-autonomous MechanismsTranssynaptic Protein InteractionsSlice Culture PreparationElectroporator SetupPipette PreparationSlice Culture IsolationTarget Cell Electroporation

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