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Biology

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使用 4 维(x、y、z 和 +)高光谱 FRET 成像和分析测量活细胞中的三维 cAMP 分布
 
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使用 4 维(x、y、z 和 +)高光谱 FRET 成像和分析测量活细胞中的三维 cAMP 分布

Article DOI: 10.3791/61720-v
October 27th, 2020

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Summary October 27th, 2020

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由于基于 Fürster 共振能量转移 (FRET) 传感器的固有的低信号与噪声比 (SNR),CAMP 信号的测量一直具有挑战性,尤其是在三个空间维度方面。在这里,我们描述了一个高光谱FRET成像和分析方法,允许测量三个空间尺寸的CAMP分布。

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