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Bioengineering
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人类诱导多能干细胞衍生心肌细胞中的单细胞光学作用潜在测量
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Please note that all translations are automatically generated.
Click here for the English version.
人类诱导多能干细胞衍生心肌细胞中的单细胞光学作用潜在测量
DOI:
10.3791/61890-v
•
08:39 min
•
December 22, 2020
•
Fitzwilliam Seibertz
2
,
Martyn Reynolds
,
Niels Voigt
2,4
1
Institute of Pharmacology and Toxicology
,
University Medical Center Goettingen, Goettingen, Germany
,
2
DZHK (German Center for Cardiovascular Research)
,
Partner Site Goettingen, Germany
,
3
Cairn Research Ltd, Faversham, United Kingdom
,
4
Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC)
,
University of Goettingen, Germany
Chapters
00:05
Introduction
00:45
Cell Preparation
01:35
Microscope Setup
03:28
Voltage Sensitive Dye Cell Loading
04:24
Electrical Field Stimulation
04:59
Optical Action Potential (AP) Acquisition
06:01
Data Analysis
06:55
Results: Representative Optical AP Profiles of Isolated Native Murine and Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
07:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们使用高速模块化光测量系统描述从诱导多能干细胞衍生心肌细胞中获得的光学采集和作用潜力的特征。
Tags
Single-cell
Optical Action Potential
Induced Pluripotent Stem Cell-derived Cardiomyocytes
Patch Clamp
High Throughput
Toxicity Screening
Comprehensive In Vitro Proarrhythmia Assay
Voltage Sensitive Dye Imaging
Epifluorescence Microscope
LED
PMT Detector
Data Acquisition
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