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מדידה פוטנציאלית לפעולה אופטית חד-תאית בקרדיומיוציטים שמקורם בתאי גזע מושרים על ידי תאי גזע אנושיים
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1Institute of Pharmacology and Toxicology,University Medical Center Goettingen, Goettingen, Germany, 2DZHK (German Center for Cardiovascular Research),Partner Site Goettingen, Germany, 3Cairn Research Ltd, Faversham, United Kingdom, 4Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC),University of Goettingen, Germany
Chapters
- 00:05Introduction
- 00:45Cell Preparation
- 01:35Microscope Setup
- 03:28Voltage Sensitive Dye Cell Loading
- 04:24Electrical Field Stimulation
- 04:59Optical Action Potential (AP) Acquisition
- 06:01Data Analysis
- 06:55Results: Representative Optical AP Profiles of Isolated Native Murine and Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
- 07:59Conclusion
